P-050 Using a second sample of ejaculate for IVF treatment

Abstract Study question Is it beneficial to use the second sample of ejaculate collected within 2 hours from previous fertilize oocytes in IVF treatments? Summary answer A significantly higher clinical pregnancy rate was achieved using ejaculate. What is known already The WHO recommends abstinence 7 days prior semen collection for standard evaluation. Although volume and sperm concentration may increase with prolonged abstinence, such have a negative impact on motility viability. Previous studies shown that following short period exhibited improved concentration, progressive motility, vitality, decreased DNA fragmentation. It suggested 1 h might be preferable ART procedures. To best our knowledge has not yet been clinically implemented routine treatment cycles. design, size, duration retrospective study couples treated at unit between Dec 2020 2022. All male partners were asked give 2-3 after first. In all cases we used fertilization whether better or worse than first one. Participants/materials, setting, methods included 422 IVF. Patients divided into groups. Group 1- 223 provided 2- 199 patients unwilling detained refused provide an additional sample. Sperm count, percentile recorded each Fertilization, cleavage, positive beta-hCG rates adjusted according maternal paternal age. Main results role chance Of samples showed quality 123 samples, identical parameters 31samples inferior 69 compared Regardless quality, only participants. Demographic characteristics as age, cause infertility, similar both Paternal age (37.2±7.0 35.2±6.3, p = 0.002 respectively). Clinical 36.9% 21.8% (p < 0.003). Higher remained adjustment OR 1.92; 95% (1.15-3.19) even when Limitations, reasons caution limitation stems nature fact groups selection bias unaware of. Wider implications findings These suggest routinely ask partners, among undergoing IVF, Further showing are needed validate these findings. Trial registration number CMC-0017-22